Which enzymes are not used for isolation of the DNA?
I. Cellulose
II. Lysozyme
III. Dnase
IV. Lyase

Options

A. I, III
B. II, III
C. III, IV
D. IV, II

Answer

To determine which enzymes are not used for the isolation of DNA, we will analyze each of the options provided:

1. **Cellulose**: This enzyme is used for the isolation of DNA from plant cells. Cellulose helps in breaking down the cell wall, allowing the release of DNA. Therefore, cellulose is involved in DNA isolation.

2. **Lysozyme**: This enzyme is used for the isolation of DNA from animal cells. Lysozyme breaks down the peptidoglycan layer in bacterial cell walls, facilitating the release of DNA. Thus, lysozyme is also involved in DNA isolation.

3. **DNase**: DNase (Deoxyribonuclease) is an enzyme that hydrolyzes DNA by breaking the phosphodiester bonds between nucleotides. Since DNase breaks down DNA, it is not used in the isolation process. Therefore, DNase is not involved in DNA isolation.

4. **Lyase**: Lyases are enzymes that catalyze the breaking of various chemical bonds by means other than hydrolysis and oxidation. They are not specifically used for isolating DNA. Thus, lyase is also not involved in DNA isolation.

From the analysis above, we conclude that the enzymes not used for the isolation of DNA are **DNase** and **Lyase**.

### Final Answer:
**DNase (III) and Lyase (IV) are not used for the isolation of DNA.**