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Which of the following statement are cor...

Which of the following statement are correct for the enzyme Taq polymerase?
(i) It remains active during the high temperature .
(ii) It requies primers for carrying out the process of polymerization.
(iii) It induces denaturation of double-stranded DNA.

A

(i) and (ii)

B

(i) and (ii)

C

(i),(ii) and (iii)

D

(ii) and (iii)

Text Solution

AI Generated Solution

The correct Answer is:
To determine which statements about Taq polymerase are correct, let's analyze each statement one by one: 1. **Statement (i): It remains active during high temperature.** - Taq polymerase is a heat-stable enzyme that is derived from the bacterium Thermus aquaticus. This enzyme is specifically designed to withstand the high temperatures used in the polymerase chain reaction (PCR). During PCR, the temperature is raised to denature the DNA, and Taq polymerase remains active and functional at these high temperatures. Therefore, this statement is **correct**. 2. **Statement (ii): It requires primers for carrying out the process of polymerization.** - Taq polymerase synthesizes new DNA strands by adding nucleotides to a primer. Primers are short sequences of nucleotides that provide a starting point for DNA synthesis. Without primers, Taq polymerase cannot initiate the polymerization process. Thus, this statement is also **correct**. 3. **Statement (iii): It induces denaturation of double-stranded DNA.** - Taq polymerase does not induce denaturation of DNA. Instead, denaturation is a separate step in the PCR process where the double-stranded DNA is heated to separate it into two single strands. Taq polymerase functions after denaturation to extend the primers and synthesize new DNA strands. Therefore, this statement is **incorrect**. Based on the analysis: - **Correct Statements**: (i) and (ii) - **Incorrect Statement**: (iii) Thus, the correct answer is that statements (i) and (ii) are correct regarding Taq polymerase. ### Summary of Correct Statements: - (i) It remains active during high temperature. **(Correct)** - (ii) It requires primers for carrying out the process of polymerization. **(Correct)** - (iii) It induces denaturation of double-stranded DNA. **(Incorrect)**
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