The construction of the first recombinant DNA emerged with the idea of linking an antibiotic resistant gene with a native plasmid of _____________________________

Options

A. Salmonella typhimurium
B. Escherichia coli
C. Streptococcus pneumoniae
D. Klebsiella pneumoniae

Answer

To solve the question regarding the construction of the first recombinant DNA, we need to identify the organism associated with the native plasmid that was used. Here’s a step-by-step breakdown of the solution:

### Step 1: Understanding Recombinant DNA
Recombinant DNA technology involves combining DNA from different sources. This is often done to study genes or to produce proteins.

### Step 2: Identifying the Key Components
The question mentions an "antibiotic resistant gene" and a "native plasmid." The antibiotic resistant gene is crucial for the construction of recombinant DNA.

### Step 3: Recognizing the Organisms Involved
The video transcript mentions two important organisms:
- **E. coli**: This bacterium is commonly used in genetic engineering as a host for plasmids.
- **Salmonella typhimurium**: This organism is specifically mentioned as having a native plasmid that was linked with the antibiotic resistant gene.

### Step 4: Linking the Antibiotic Resistant Gene
The antibiotic resistant gene was linked to the native plasmid of **Salmonella typhimurium**. This is the key point that answers the question.

### Final Answer
The construction of the first recombinant DNA emerged with the idea of linking an antibiotic resistant gene with a native plasmid of **Salmonella typhimurium**.

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