To produce insulin via a non-human cell, which of the following would be required to be inserted in the vector's plasmid?

Options

A. a segment of DNA from a human
B. a segment of DNA from another becterium
C. a molecule of insulin
D. an enzyme

Answer

To solve the question of which component is required to be inserted into the vector's plasmid for the production of insulin via a non-human cell, we can follow these steps:

### Step-by-Step Solution:

1. **Understanding the Requirement**:
- The goal is to produce insulin using a non-human cell. This involves genetic engineering where a specific gene is inserted into a plasmid vector.

2. **Identifying the Insulin Gene**:
- Insulin is a protein that is produced from a specific gene found in human DNA. Therefore, we need to identify the correct segment of DNA that codes for insulin.

3. **Selecting the Correct DNA Segment**:
- The DNA segment that codes for insulin must be derived from human pancreatic cells since insulin is naturally produced in the pancreas.

4. **Vector Plasmid Insertion**:
- The identified segment of DNA (the insulin gene) will be inserted into a plasmid vector. This vector will then be introduced into a non-human cell (like bacteria) to facilitate the production of insulin.

5. **Evaluating the Options**:
- A segment of DNA from a human: This is the correct option as it contains the insulin gene.
- A segment of DNA from another bacterium: This would not produce insulin since it does not contain the human insulin gene.
- A molecule of insulin: This is not a DNA segment and cannot be inserted into the plasmid.
- An enzyme: While enzymes are important in biological processes, they are not the genetic material needed for insulin production.

6. **Conclusion**:
- The correct answer is **A**: A segment of DNA from a human.