Which of the following show a correct pair with respect to pBR322 ?

Options

A. ori - Hind III
B. `tet^R` -BamHI, Sal I
C. `amp^R` - Pst , Pvu II
D. rop - Pvu I

Answer

To solve the question regarding the correct pair with respect to pBR322, we will analyze the components and connections of the pBR322 plasmid.

### Step-by-Step Solution:

1. **Understand pBR322 Structure**:
- pBR322 is a plasmid that contains genes for antibiotic resistance, specifically for ampicillin and tetracycline.
- It has various restriction sites for enzymes like BamHI, SalI, PSTI, and PVU1.

2. **Identify Antibiotic Resistance Genes**:
- The plasmid has two main antibiotic resistance genes:
- **Ampicillin resistance (bla)**: This allows for the selection of bacteria that can grow in the presence of ampicillin.
- **Tetracycline resistance (tet)**: This allows for the selection of bacteria that can grow in the presence of tetracycline.

3. **Analyze the Given Options**:
- The question asks for a correct pair with respect to pBR322.
- The options provided include pairs of restriction enzymes and the antibiotics they are associated with.

4. **Check the Connections**:
- According to the transcript, BamHI and SalI are associated with tetracycline resistance.
- PSTI is connected to ampicillin resistance but is incorrectly stated to connect with PVU1 instead of PVU2.

5. **Conclusion**:
- The correct pair with respect to pBR322 is **tetracycline with BamHI and SalI**.

### Final Answer:
The correct pair with respect to pBR322 is **Tetracycline: BamHI and SalI**.

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