Consider the reactions and arrange the correct sequence with respect to glycolysis
i Formation of fructose 1,6 biosphere
ii Dehydrogenation
iii cleavage to from triose - 3 phosphate
iv second substrate - level phosphorylation
v Dehydration

Options

A. `irarriirarriiirarrivrarrv`
B. `irarriiirarriirarrvrarriv`
C. `irarriirarriiirarrvrarriv`
D. `irarriiirarriirarrivrarrv`

Answer

To arrange the steps of glycolysis in the correct sequence based on the given reactions, we will follow the glycolytic pathway step by step.

### Step-by-Step Solution:

1. **Formation of Fructose 1,6-bisphosphate (i)**:
- The glycolysis process begins with glucose being phosphorylated to form glucose-6-phosphate, which is then converted into fructose-6-phosphate. The addition of another phosphate group (from ATP) leads to the formation of fructose-1,6-bisphosphate.

2. **Cleavage to form Triose-3-phosphate (iii)**:
- The fructose-1,6-bisphosphate is then cleaved into two three-carbon molecules: glyceraldehyde-3-phosphate (G3P) and dihydroxyacetone phosphate (DHAP). This step is crucial as it generates the triose phosphates.

3. **Dehydrogenation (ii)**:
- The next step involves the oxidation of glyceraldehyde-3-phosphate (G3P) to form 1,3-bisphosphoglycerate. During this reaction, NAD+ is reduced to NADH, which involves the removal of hydrogen ions (dehydrogenation).

4. **Dehydration (v)**:
- The conversion of 2-phosphoglycerate to phosphoenolpyruvate (PEP) involves the removal of a water molecule, which is termed dehydration.

5. **Second Substrate-level Phosphorylation (iv)**:
- Finally, phosphoenolpyruvate is converted to pyruvate, and in this step, ATP is generated through substrate-level phosphorylation.

### Correct Sequence:
Based on the steps outlined, the correct sequence with respect to glycolysis is:
1. Formation of Fructose 1,6-bisphosphate (i)
2. Cleavage to form Triose-3-phosphate (iii)
3. Dehydrogenation (ii)
4. Dehydration (v)
5. Second Substrate-level Phosphorylation (iv)

### Final Arrangement:
i → iii → ii → v → iv