A set of 6 test tube having same temperature but different pH were setup to conduct an experiment . To each of the tubes, starch and amylase were added in a mix of sterile water . The concentration was kept the same . The following table notes the reaction completion time for each of the six tubes.
`{:(PH,1,3,5,7,9,11),("Time taken to complete in seconds (s).",94,64,32,5,35,66):}`
Using the above data , determine the pH where amylase has shown highest rate of activity.

Options

A. pH 1
B. pH 5
C. pH 7
D. pH 11

Answer

To determine the pH at which amylase shows the highest rate of activity, we will analyze the provided data regarding the time taken for the reaction to complete at different pH levels.

### Step-by-Step Solution:

1. **Understand the Data**: We have 6 test tubes with different pH levels and the time taken for the reaction to complete is recorded. The pH levels and corresponding times are:
- pH 1: 94 seconds
- pH 3: 64 seconds
- pH 5: 32 seconds
- pH 7: 5 seconds
- pH 9: 35 seconds
- pH 11: 66 seconds

2. **Identify the Reaction Completion Time**: We need to find out which pH resulted in the least time taken for the reaction to complete. The lower the time, the higher the rate of activity of the enzyme amylase.

3. **Compare the Times**:
- pH 1: 94 seconds
- pH 3: 64 seconds
- pH 5: 32 seconds
- pH 7: 5 seconds (least time)
- pH 9: 35 seconds
- pH 11: 66 seconds

4. **Determine the Optimal pH**: From the comparison, we can see that at pH 7, the reaction completed in just 5 seconds, which is significantly lower than the other pH levels.

5. **Conclusion**: Therefore, the pH where amylase has shown the highest rate of activity is **pH 7**.

### Final Answer:
The pH where amylase has shown the highest rate of activity is **pH 7**.