Read the following statement regarding DNA recombinant technology. Identify the statements that are true and false and select the appropriate option.
I. Selectable marker helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants.
II. Plasmids have the ability to replicate within bacterial cells independent of the control of chromosomal DNA making it a suitable cloning vector.
III. The DNA fragments for isolation can be separated by a technique known as thin-layer chromatography.
IV. A technique where cells are bombarded with high velocity micro-particles of Au or W coated with DNA is known as micro-injection.
`{:(,I,II,III,IV),(1,"False","False","True","True"),(2,"True","True","False","False"),(3,"True","True","True","True"),(4,"False","False","True","False"):}`

Options

A. 1
B. 2
C. 3
D. 4

Answer

To solve the question regarding the statements on DNA recombinant technology, we will analyze each statement one by one to determine if they are true or false.

### Step 1: Analyze Statement I
**Statement I:** "Selectable marker helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants."
- **Analysis:** This statement is true. Selectable markers are essential in recombinant DNA technology as they allow for the identification of cells that have taken up the recombinant DNA (transformants) and the elimination of those that have not (non-transformants).

### Step 2: Analyze Statement II
**Statement II:** "Plasmids have the ability to replicate within bacterial cells independent of the control of chromosomal DNA making it a suitable cloning vector."
- **Analysis:** This statement is also true. Plasmids are extrachromosomal DNA molecules that can replicate independently of the bacterial chromosomal DNA, which makes them ideal vectors for cloning.

### Step 3: Analyze Statement III
**Statement III:** "The DNA fragments for isolation can be separated by a technique known as thin-layer chromatography."
- **Analysis:** This statement is false. DNA fragments are typically separated using gel electrophoresis, not thin-layer chromatography. Thin-layer chromatography is more commonly used for separating small molecules.

### Step 4: Analyze Statement IV
**Statement IV:** "A technique where cells are bombarded with high velocity micro-particles of Au or W coated with DNA is known as micro-injection."
- **Analysis:** This statement is false. The technique described is known as "gene gun" or biolistics, not micro-injection. Micro-injection involves directly injecting DNA into the cell using a fine needle.

### Conclusion
Now that we have analyzed all the statements, we can summarize their truth values:
- Statement I: True
- Statement II: True
- Statement III: False
- Statement IV: False

The correct option based on the analysis is:
**Option 2: "True, True, False, False"**