Klinefelter's syndrome can be easily identified by

Options

A. PCR
B. Karyotype
C. ELISA
D. RFLP

Answer

To solve the question regarding how Klinefelter's syndrome can be easily identified, we will analyze each option provided and determine which method is appropriate for identifying this genetic condition.

### Step-by-Step Solution:

1. **Understanding Klinefelter's Syndrome**:
- Klinefelter's syndrome is a genetic condition that occurs in males when they have an extra X chromosome, leading to a karyotype of 47,XXY instead of the typical 46,XY.

2. **Evaluating the Options**:
- **Option 1: PCR (Polymerase Chain Reaction)**:
- PCR is a technique used to amplify small segments of DNA. While it is useful in many genetic analyses, it does not provide a direct method for identifying chromosomal abnormalities like Klinefelter's syndrome.
- **Conclusion**: This option is incorrect.

- **Option 2: Karyotype**:
- A karyotype involves taking photographs of chromosomes during cell division, which allows for the arrangement and analysis of chromosomes. This method can clearly show the presence of an extra X chromosome in males with Klinefelter's syndrome.
- **Conclusion**: This option is correct.

- **Option 3: ELISA (Enzyme-Linked Immunosorbent Assay)**:
- ELISA is a technique used to detect specific proteins or antibodies in a sample. It is not used for identifying chromosomal abnormalities.
- **Conclusion**: This option is incorrect.

- **Option 4: RFLP (Restriction Fragment Length Polymorphism)**:
- RFLP is a technique used for genetic testing to identify variations in DNA sequences. It does not provide a direct method for identifying chromosomal abnormalities like Klinefelter's syndrome.
- **Conclusion**: This option is incorrect.

3. **Final Answer**:
- The method that can easily identify Klinefelter's syndrome is **Karyotype** (Option 2).