Mark the incorrect differences between eukaryotic and prokaryotic transcription.
I. In eukaryotes , the genes are split having exons and introns while in prokaryotes it isn't .
ii The structural gene which is to be transcribed is generally mono- cistronic in prokaryotes and poly-cistronic in eukaryotes .
III . The RAN formed with the help of RNA polymerase in eukaryotes requires future processing to function as m-RNA while in prokaryotes it is directly used as m - RNA.
IV. In eukaryotes, RNA polymerase binds to the promoter region while in prokaryotes it binds to the operator region .

Options

A. I and IV
B. II and III
C. II and IV
D. III and IV

Answer

To solve the question regarding the incorrect differences between eukaryotic and prokaryotic transcription, we will analyze each statement one by one.

### Step-by-Step Solution:

1. **Statement I**: "In eukaryotes, the genes are split having exons and introns while in prokaryotes it isn't."
- **Analysis**: This statement is correct. Eukaryotic genes contain both exons (coding regions) and introns (non-coding regions), while prokaryotic genes do not have introns; they are continuous coding sequences.

2. **Statement II**: "The structural gene which is to be transcribed is generally mono-cistronic in prokaryotes and poly-cistronic in eukaryotes."
- **Analysis**: This statement is incorrect. In prokaryotes, genes are often polycistronic, meaning a single mRNA molecule can encode multiple proteins. In contrast, eukaryotic mRNAs are typically monocistronic, meaning they encode only one protein.

3. **Statement III**: "The RNA formed with the help of RNA polymerase in eukaryotes requires future processing to function as mRNA while in prokaryotes it is directly used as mRNA."
- **Analysis**: This statement is correct. In eukaryotes, the primary RNA transcript undergoes processing (capping, polyadenylation, and splicing) before it becomes mature mRNA. In prokaryotes, the RNA produced is immediately functional as mRNA without such processing.

4. **Statement IV**: "In eukaryotes, RNA polymerase binds to the promoter region while in prokaryotes it binds to the operator region."
- **Analysis**: This statement is incorrect. In both eukaryotes and prokaryotes, RNA polymerase binds to the promoter region to initiate transcription. The operator region is involved in the regulation of transcription in prokaryotes but is not the binding site for RNA polymerase.

### Conclusion:
The incorrect statements are II and IV. Therefore, the answer to the question is that the incorrect differences between eukaryotic and prokaryotic transcription are found in statements II and IV.

### Final Answer:
The incorrect differences are in statements II and IV.

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