During glycolysis , water is released along with the formation of

Options

A. 3- phosphoglyceric acid
B. Phosphoenol pyruvic acid
C. Dihydroxy acetone phosphate
D. 3- phosphoglyceric acid

Answer

### Step-by-Step Solution:

1. **Understanding Glycolysis**: Glycolysis is the metabolic pathway that converts glucose into pyruvate, releasing energy and producing ATP in the process. It occurs in the cytoplasm of cells and consists of ten enzymatic reactions.

2. **Initial Steps**: The process begins with the phosphorylation of glucose to form glucose-6-phosphate, which uses one molecule of ATP. This is followed by the conversion of glucose-6-phosphate to fructose-6-phosphate, which also consumes one ATP.

3. **Formation of Fructose 1,6-bisphosphate**: Fructose-6-phosphate is then converted into fructose-1,6-bisphosphate, which is an important intermediate in glycolysis.

4. **Splitting of Fructose 1,6-bisphosphate**: The fructose-1,6-bisphosphate is split into two three-carbon molecules: glyceraldehyde-3-phosphate (G3P) and dihydroxyacetone phosphate (DHAP).

5. **Conversion to 1,3-bisphosphoglycerate**: G3P is then converted into 1,3-bisphosphoglycerate, which involves the reduction of NAD+ to NADH.

6. **Formation of 2-Phosphoglycerate**: The 1,3-bisphosphoglycerate is converted into 2-phosphoglycerate, and during this conversion, a water molecule is released.

7. **Conversion to Phosphoenolpyruvate**: The 2-phosphoglycerate is then converted into phosphoenolpyruvate (PEP). This step also involves the release of a water molecule.

8. **Final Product - Pyruvate**: Finally, phosphoenolpyruvate is converted into pyruvate, which is the end product of glycolysis.

9. **Conclusion**: During glycolysis, water is released during the conversion of 2-phosphoglycerate to phosphoenolpyruvate. Therefore, water is released along with the formation of phosphoenolpyruvate.

### Final Answer:
Water is released along with the formation of **Phosphoenolpyruvate** during glycolysis.

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