Which of the following is not a characteristic feature of the vector pBR322?

Options

A. It has two antibiotic - resistance genes : `amp^R and tet^R`.
B. It was the first artificial cloning vector constructed in 1997 by Boliver and Rodriguez.
C. The ampicillin-resistance gene has restriction sites for Bam HI and Sal I
D. The restriction site for Pvu II is present in the replication of lpasmid (rop) gene .

Answer

To determine which of the following is not a characteristic feature of the vector pBR322, we need to analyze the given statements regarding this cloning vector.

### Step-by-Step Solution:

1. **Understanding pBR322**:
- pBR322 is a well-known plasmid vector used in molecular cloning. It was developed by Bolivar and Rodriguez in 1977.

2. **Identifying Features of pBR322**:
- **Cloning Sites**: pBR322 contains multiple cloning sites (MCS) that allow for the insertion of foreign DNA.
- **Selectable Markers**: It has two antibiotic resistance genes: one for ampicillin (amp^R) and one for tetracycline (tet^R). These genes allow for the selection of successfully transformed bacteria.
- **Origin of Replication (ORI)**: pBR322 has a specific origin of replication that allows it to replicate independently within a host cell.

3. **Evaluating Each Statement**:
- **Statement 1**: "It has ampicillin and tetracycline resistance genes."
- This is correct; pBR322 has both resistance genes.
- **Statement 2**: "It was the first artificial cloning vector constructed in 1997 by Bolivar and Rodriguez."
- This statement is incorrect because pBR322 was constructed in 1977, not 1997.
- **Statement 3**: "Ampicillin-resistant gene has restriction sites for BAMH1, ACHI1, and SALFAST."
- This statement is incorrect; the restriction site for BAMH1 is actually located in the tetracycline resistance gene, not in the ampicillin resistance gene.
- **Statement 4**: "The restriction site for PPVU2 is present in the replication of plasmid ROP gene."
- This statement is correct; the ROP gene is involved in the regulation of plasmid replication.

4. **Conclusion**:
- The statement that is not a characteristic feature of pBR322 is the one claiming it was constructed in 1997.

### Final Answer:
The statement that is not a characteristic feature of the vector pBR322 is: "It was the first artificial cloning vector constructed in 1997 by Bolivar and Rodriguez."

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