Where will be the human insulin found in the genetically altered prokaryote ?

Options

A. Genetically engineered bacterial cytoplasm
B. Genetically engineered bacterial nucleus
C. Human liver having the modified bacteria
D. Human pancreas having the modified bacteria

Answer

To solve the question of where human insulin will be found in a genetically altered prokaryote, we can follow these steps:

### Step-by-Step Solution:

1. **Understanding Genetic Engineering**:
- Genetic engineering involves inserting a gene of interest (in this case, the human insulin gene) into a host organism, which can be a prokaryote like E. coli.

2. **Insertion of Insulin Gene**:
- The human insulin gene is inserted into a plasmid (a small circular DNA molecule) using techniques such as restriction enzymes and ligases.

3. **Transformation of Bacteria**:
- The plasmid containing the insulin gene is then introduced into the bacterial cells (like E. coli) through a process called transformation.

4. **Protein Production**:
- Once the bacteria take up the plasmid, they replicate it along with their own DNA. The insulin gene is then expressed, leading to the production of insulin protein.

5. **Location of Protein Synthesis**:
- In prokaryotes (like bacteria), protein synthesis occurs in the cytoplasm. Therefore, the human insulin produced in genetically altered bacteria will be found in the cytoplasm of these bacteria.

6. **Conclusion**:
- Based on the above steps, the correct answer to the question is that human insulin will be found in the **genetically engineered bacterial cytoplasm**.

### Final Answer:
**Option A: Genetically engineered bacterial cytoplasm.**

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