In Miller's experiment, ……... Was not used

Options

A. Methane
B. Hydrogen
C. Water vapour
D. Nitrogen

Answer

**Step-by-Step Solution:**

1. **Understanding Miller's Experiment**: The experiment conducted by Stanley Miller in 1953 aimed to simulate the conditions of early Earth to test the hypothesis of chemical evolution, which suggests that life could arise from non-living organic molecules.

2. **Identifying the Gases Used**: In Miller's experiment, he used a mixture of gases that were believed to represent the early Earth's atmosphere. The gases included:
- Methane (CH₄)
- Hydrogen (H₂)
- Water vapor (H₂O)
- Ammonia (NH₃)

3. **Analyzing the Options**: The question asks which gas was not used in Miller's experiment. The options provided are:
- Methane
- Hydrogen
- Water vapors
- Nitrogen

4. **Determining the Incorrect Option**: From the gases listed above, we can see that:
- Methane was used.
- Hydrogen was used.
- Water vapor was used.
- **Nitrogen (N₂)** was not used in the experiment.

5. **Conclusion**: Therefore, the gas that was not used in Miller's experiment is **Nitrogen**.

**Final Answer**: Nitrogen was not used in Miller's experiment.

---