Majority of proteins ( of intracellular use ) are not synthesized on :
A -Ribosomes produced in the nucleolus
B - Ribosomes attached with ER and nuclear membrane
C - Free ribosomes of eukaryote cells
D - Ribosomal sub- units forming polyribosomes
E - Ribosomal sub- units forming polyribosomes Which are found in peri-mitochondrial space of prokaryotic cells

Options

A. A and D
B. B,C and D
C. D and E
D. A , C and E

Answer

To solve the question regarding the synthesis of proteins for intracellular use, we need to analyze each of the provided options carefully.

### Step-by-Step Solution:

1. **Understanding the Question**:
The question asks which of the following options does not synthesize the majority of proteins used intracellularly.

2. **Analyzing Option A**:
- **Ribosomes produced in the nucleolus**: Ribosomes are indeed produced and assembled in the nucleolus. They play a crucial role in protein synthesis. Therefore, this option is not correct as ribosomes from the nucleolus do synthesize proteins.

3. **Analyzing Option B**:
- **Ribosomes attached with ER and nuclear membrane**: Ribosomes that are attached to the rough endoplasmic reticulum (ER) and the nuclear membrane are involved in synthesizing proteins that are either secreted or incorporated into membranes. Hence, this option also does not fit the criteria as they do synthesize proteins.

4. **Analyzing Option C**:
- **Free ribosomes of eukaryotic cells**: Free ribosomes in eukaryotic cells synthesize proteins that function within the cytosol. This option is also incorrect since they do synthesize intracellular proteins.

5. **Analyzing Option D**:
- **Ribosomal subunits forming polyribosomes**: Polyribosomes (or polysomes) consist of multiple ribosomes translating a single mRNA strand simultaneously. This is a common mechanism for synthesizing proteins. Therefore, this option does not meet the criteria either.

6. **Analyzing Option E**:
- **Ribosomal subunits forming polyribosomes found in peri-mitochondrial space of prokaryotic cells**: This option is misleading. Prokaryotic cells do not have a peri-mitochondrial space as they lack mitochondria. Thus, this option is incorrect as it suggests a structure that does not exist in prokaryotes.

### Conclusion:
After analyzing all options, the majority of proteins for intracellular use are not synthesized on **Option E**: Ribosomal subunits forming polyribosomes found in peri-mitochondrial space of prokaryotic cells.

### Final Answer:
**E - Ribosomal sub-units forming polyribosomes which are found in peri-mitochondrial space of prokaryotic cells.**