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Restriction endonuclease ( EcoRI) enzyme...

Restriction endonuclease ( EcoRI) enzyme cut the strand of DNA

A

From the centre of the palindromic sequence .

B

a little away from the centre of the palindromic sequence

C

a little away from recognization site.

D

cuts only single-stranded DNA

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The correct Answer is:
A
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Which of the followoing restriction endonuclease enzyme produce blunt end in DNA :

Restriction endonuclease is employed for cutting

Correct statement about restriction endonuclease. (I) It is the enzyme which cuts DNA at a particular point. (II) The first restriction endonuclease was Hindll. (III) Overall,900 restriction enzymes have been isolated from about 200 strains. (IV) Each restriction endonuclease recognizes a specific palindromic sequence.

With the help of diagrams show the different steps in the formation of reeombinant DNA by action of restriction endonuclease enzyme EcoRI. Name the technique that 18 used for separating the fragments of DNA cut by restriction endonncleases.

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