Given below are some step for slicing foreign DNA into a plasmid and then inserting the plasmid into a bacterium.
(i) Transform bacteria with recombinant
(ii) Cut the plasmid DNA using restriction enzyme .
(iii) Extract plasmid DNA to - DNA fragments DNA
(iv) Bind the plasmid DNA to non-plasmid DNA fragments .
(v) Use ligase to seal plasmid DNA to non- plasmid DNA
Arrange the above steps in the right sequence and select the CORRECT option

Options

A. iv, v, ii, iii , iv
B. iii , ii , iv ,v, i
C. iii, iv , v, i, ii
D. ii, iii, v, iv ,i

Answer

To arrange the steps for slicing foreign DNA into a plasmid and then inserting the plasmid into a bacterium in the correct sequence, we can follow these steps:

1. **Extract plasmid DNA to obtain DNA fragments**: This is the first step where we isolate the plasmid DNA from the bacterial cells.

2. **Cut the plasmid DNA using restriction enzyme**: In this step, we use restriction enzymes to cut the plasmid DNA at specific sequences. This creates open ends in the plasmid where foreign DNA can be inserted.

3. **Bind the plasmid DNA to non-plasmid DNA fragments**: After cutting the plasmid, we can now bind the foreign DNA (non-plasmid DNA) to the plasmid DNA.

4. **Use ligase to seal plasmid DNA to non-plasmid DNA**: The enzyme ligase is then used to seal the nicks in the DNA backbone, joining the plasmid DNA and the foreign DNA together to form a recombinant DNA molecule.

5. **Transform bacteria with recombinant DNA**: Finally, we introduce the recombinant plasmid into bacterial cells through a process called transformation. This allows the bacteria to take up the plasmid and replicate it.

Thus, the correct sequence of steps is:
(iii) Extract plasmid DNA to obtain DNA fragments.
(ii) Cut the plasmid DNA using restriction enzyme.
(iv) Bind the plasmid DNA to non-plasmid DNA fragments.
(v) Use ligase to seal plasmid DNA to non-plasmid DNA.
(i) Transform bacteria with recombinant DNA.

### Correct Order:
(iii) → (ii) → (iv) → (v) → (i)