In which of the following aspect PCR is not leading over gene cloning ?

Options

A. Efficiency
B. Less user's skill
C. Error probability
D. Less expensive

Answer

To solve the question regarding the aspects in which PCR is not leading over gene cloning, we can break down the comparison between the two techniques step by step.

### Step-by-Step Solution:

1. **Understanding PCR and Gene Cloning**:
- **PCR (Polymerase Chain Reaction)**: A technique used to amplify small segments of DNA, producing millions of copies of a specific DNA sequence.
- **Gene Cloning**: A method used to create identical copies of a particular gene. It involves inserting the gene into a vector and replicating it within a host organism.

2. **Comparing Key Aspects**:
- **Efficiency**: PCR is generally more efficient in amplifying DNA quickly and producing a large number of copies from a small amount of starting material.
- **User Skill Requirement**: PCR is relatively straightforward and can be performed with basic training, while gene cloning requires more advanced skills and knowledge.
- **Error Probability**: PCR has a higher error rate due to the amplification process, which can introduce mutations. In contrast, gene cloning typically results in fewer errors, producing exact copies of the original gene.
- **Cost**: PCR is usually less expensive than gene cloning, as it requires fewer resources and less time.

3. **Identifying the Aspect Where PCR is Not Leading**:
- From the comparison, we can see that the aspect where PCR does not lead over gene cloning is **error probability**. Gene cloning has a lower error rate, producing more accurate and defect-free copies of the gene.

### Conclusion:
The correct answer to the question is that PCR is not leading over gene cloning in terms of **error probability**.

---