With respect to RFLP , arrange the following sentences in order .
I. Agarose gel electrophoresis
II. Isolation on DNA
III . Cutting with Restriction Enzyme
IV. PCR

Options

A. I, II, III, IV
B. II, I, IV, III
C. II, III, IV, I
D. II, IV, III, I

Answer

To arrange the sentences in the correct order with respect to RFLP (Restriction Fragment Length Polymorphism), we can follow these steps:

1. **Isolation of DNA (II)**: The first step in RFLP analysis is to isolate the DNA from the cells or tissue samples. This is essential as we need a pure sample of DNA to work with.

2. **PCR (IV)**: After isolating the DNA, the next step is to amplify the DNA using Polymerase Chain Reaction (PCR). This technique allows us to create multiple copies of the specific DNA segment we are interested in analyzing.

3. **Cutting with Restriction Enzyme (III)**: Once we have enough DNA, we then use restriction enzymes to cut the DNA at specific sequences. This creates fragments of varying lengths, which is crucial for the RFLP analysis.

4. **Agarose Gel Electrophoresis (I)**: Finally, we perform agarose gel electrophoresis to separate the DNA fragments based on their sizes. This allows us to visualize the different lengths of the fragments, which can then be analyzed for polymorphisms.

Thus, the correct order of the sentences is:
II → IV → III → I

### Final Arrangement:
- II. Isolation of DNA
- IV. PCR
- III. Cutting with Restriction Enzyme
- I. Agarose gel electrophoresis