If the sequence of bases in sense strand of DNA is 5'-GTTCATCG-3', then the sequence of bases in its RNA transcript would be-

Options

A. 5'-GTTCATCG-3'
B. 5'-GUUCAUCG-3'
C. 5'-CAAGTAGC-3'
D. 5'-CAAGUAGC-3'

Explanation

To determine the RNA transcript from the given sense strand of DNA, follow these steps:

### Step 1: Identify the Sense Strand
The given sense strand of DNA is:
5'-GTTCATCG-3'

### Step 2: Determine the Antisense Strand
The antisense strand is complementary to the sense strand. To find the antisense strand, we pair the bases as follows:
- G (Guanine) pairs with C (Cytosine)
- T (Thymine) pairs with A (Adenine)
- C (Cytosine) pairs with G (Guanine)
- A (Adenine) pairs with T (Thymine)

So, the complementary sequence (antisense strand) will be:
3'-CAAGTAGC-5'

### Step 3: Transcription Process
During transcription, the RNA polymerase reads the antisense strand from 3' to 5' and synthesizes RNA from 5' to 3'. The RNA bases are complementary to the antisense strand, with the exception that uracil (U) replaces thymine (T).

### Step 4: Write the RNA Transcript
Now, we will write the RNA transcript based on the antisense strand:
- C (from antisense) pairs with G (in RNA)
- A (from antisense) pairs with U (in RNA)
- A (from antisense) pairs with U (in RNA)
- G (from antisense) pairs with C (in RNA)
- T (from antisense) pairs with A (in RNA)
- A (from antisense) pairs with U (in RNA)
- G (from antisense) pairs with C (in RNA)
- C (from antisense) pairs with G (in RNA)

Thus, the RNA transcript will be:
5'-GUUCAUCGA-3'

### Step 5: Final Answer
The sequence of bases in the RNA transcript is:
**5'-GUUCAUCGA-3'**