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The restriction endonuclease EcoR-I reco...

The restriction endonuclease EcoR-I recognizes and cleaves DNA sequence as shown below
`"5' -G A A T T C-3'"`
`"3' -C T T A A G'5'"`
What is the probable number of cleavage sites that can occur in a 10 kb long random DNA sequence?

A

10

B

2

C

100

D

50

Text Solution

Verified by Experts

The correct Answer is:
B
Eco RI is an example of six-cutter restriction endonuclease. It usually cleaves once in every 4096 bp.
`((1)/(4))^(6)=(1)/(4096)`
Given length of DNA fragment = 10 Kb = 10000 bp
Hence,
Probable no. of cleaving sites `=(10000)/(4096)=2.44`
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