What procedure do you follow to understand anaerobic respiration in your school laboratory ?

Explanation

Aim:To prove that `CO_2` is released during anaerobic respiration .
Apparatus : Thermos flask , splitted corks , thermometer , wash bottle, glass tubes , liquid paraffin, glucose solution , yeast cells , bicarbonate solution, Janus Green B solution.
Procedure :
(1)Heat the glucose solution in a beaker , pour it in a thermos flask
(2)To remove dissolved oxygen from glucose solution by boiling it in thermos flask for a minute and then cooling it without shaking.
(3)Now add some yeast to the glucose solution and fix two-holed rubber stopper to the flask .
(4)The supply of oxygen from the air can be cut off by pouring a 1 cm layer of liquid paraffin on the mixture (Thermos is heat resistant can't be used for boiling).
(5)Insert one end of the thermometer into the thermos flask. See the end of thermometer kept inside the solution.
(6)Arrange for any gas produced by the yeast to escape through a wash bottle containing bicarbonate solution or lime water as shown in the figure.
(7)Add a few drops of diazine green (Janus Green B) solution to the yeast suspension before you pour liquid paraffin over it.
(8)The blue diazine green solution turns pink when oxygen is in short supply around it .
(9)Warm the apparatus to about `37^@` F in order to speed up the test . (If you do experiment in test tube).
(10)Keep the apparatus undisturbed for one or two days
Observations :
(1)After two days if can be observed that lime water of the wash bottle turns into milky white precipitate.
(2)Increase in the temperature noted on yeast cells respire and release energy .
(3)Alcohol smell given off from the flask.
Result : These observations indicate that yeast cells respire anaerobically converting glucose solution into `CO_2` , ethyl alcohol and releasing heat energy .