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Restriction endonuclease is employed for...

Restriction endonuclease is employed for cutting

A

A single stranded DNA

B

Double stranded DNA

C

RNA fragment

D

mRNA

Text Solution

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The correct Answer is:
B
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Knowledge Check

  • Restriction endonucleases

    A
    Cleave DNA at hgihtly specific recoginition sequences
    B
    Are inserted into bacteria by bacteeriophages
    C
    Are made only by eukaryotic cells
    D
    Add methyl groups to specific DNA sequences
  • Restriction endonucleases

    A
    Are synthesized by bacteria as part of their defense compound
    B
    Are present in mammalian cells for degradation of DNA when the cell dies
    C
    Are used in genetic engineering for ligating two DNA molecules
    D
    Are used for in vitro DNA sythesis
  • Restriction endonucleases

    A
    Are used for in utro DNA synthesis
    B
    Are synthesized by bacteria as part of defense mechanism
    C
    Are present in mammalian cells for degradation of DNA when the cells dies
    D
    Are used in genetic engineering
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    Endonuclease in employed in

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    Restriction endonuclease was isolated for the first time by W. Aber in 1962 in bacteria. Restriction endonucleases cut the DNA duplex at specific points therefore they are also called as molecular scissors or biological scissors. Three types of restriction endemicleases are Type 1. Type II and Type III but only Type II restriction endonucleases are used in recombinant DNA technology. Restriction endonuclease EcoR I recognises the base sequence GAATTC In DNA duplex and cut strands between G and A. Which type of restriction endonucleases is used most in genetic engineering?