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Enzyme required for polymerase chain rea...

Enzyme required for polymerase chain reaction (PCR) is

A

RNA polymerase

B

Ribonuclease

C

Taq polymerase

D

Endonuclease

Text Solution

AI Generated Solution

The correct Answer is:
**Step-by-Step Solution:** 1. **Understanding the Question**: The question asks for the enzyme required for the Polymerase Chain Reaction (PCR). 2. **Identifying the Options**: The options given are: - A) RNA polymerase - B) Ribonuclease - C) Taq polymerase - D) Endonuclease 3. **Analyzing Each Option**: - **RNA polymerase**: This enzyme synthesizes RNA from a DNA template but is not used in PCR. - **Ribonuclease**: This enzyme degrades RNA and is not relevant to DNA amplification in PCR. - **Taq polymerase**: This is a specific type of DNA polymerase that is heat-stable and is used in PCR to synthesize new DNA strands. - **Endonuclease**: This enzyme cuts DNA at specific sites but is not used for amplifying DNA in PCR. 4. **Selecting the Correct Answer**: Based on the analysis, Taq polymerase is the enzyme specifically required for PCR due to its thermostability, allowing it to function at the high temperatures used during the process. 5. **Conclusion**: The correct answer is **C) Taq polymerase**. ---
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Knowledge Check

  • Polymerase chain reaction (PCR) is used for

    A
    In vivo replication of specific DNA sequence using thermostable DNA polymerase
    B
    In vitro synthesis of mRNA
    C
    In vivo synthesis of mRNA
    D
    Separation of DNA fragments according to their size.
  • Which of the following enzymes is used in polymerase chain reaction (PCR) ?

    A
    Taq polymerase
    B
    Vent polymerase
    C
    Both of these
    D
    None of these
  • The function of polymerase chain reaction (PCR) is

    A
    translation
    B
    transcription
    C
    DNA amplification
    D
    None of these
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