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Gel electrophoresis is used for...

Gel electrophoresis is used for

A

Cutting DNA

B

Isolation of DNA

C

Construction of recombinant DNA

D

Separation of DNA fragments according to their size.

Text Solution

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The correct Answer is:
D
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Knowledge Check

  • Gel electrophoresis is a

    A
    technique of separation of charged molecules under the influence of magnetic field
    B
    technique of incorpotation of DNA molecules into the cell through transient pores made due to electrical impulses
    C
    technique of separation of DNA fragments through the pores of agarose gel under the influence of electric field
    D
    technique of separation and purification of gene products.

  • Assertion (A) : Agarose gel electrophoresis is used to check the progression of a restriction enzyme digestion. Reason (R) : Restriction enzyme digestions are performed by incubating purified DNA molecules with restriction enzyme.

    A
    Both assertion (A) and reason (R) are true and reason (R) is the correct explanation of assertion (A).
    B
    Both assertion (A) and reason (R) are true but reason (R) is not the correct explanation of assertion (A).
    C
    Assertion (A) is true but reason (R) is false.
    D
    Assertion (A) is false but reason (R) is true.

  • Select the correct option to fill up balnks. (i) ________is a natural polymer extracted from_____. (ii) The DNA fragments purified by gel electrophoresis are used in constructing______by joining them with_______. (iii) The ligation of alien DNA is carried out at a________. present in one of the two________in a plasmid vector. (iv)_________enzyme remains active during the high temperature induced denaturation of ds DNA (v) DNA fragments are resolved according to their_________ through ______in agarose gel electrophoresis.

    A
    (i) Agarose, sea weeds (ii) recombinant DNA, cloning vector (iii) restriction site, antibiotic resistance genes (iv) Taq polymerase (v) size, sieving effect
    B
    (i) Agarose, sea weeds (ii) Restriction site, antibiotic resistance genes (iii) recombinant DNA, cloning vector (iv) Taq polymerase (v) size, sieving effect
    C
    (i) Agarose, sea weeds (ii) restriction site, antibiotic resistance genes (iii) recombinant DNA, cloning vector (iv) Taq polymerease (v) size, sieving effect
    D
    (i) size, sieving effect (ii) agarose, eas weeds (iii) recombinant DNA cloning vector (iv) Taq polymerase (v) restriction site, antibiotic resistance genes

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    Gel electrophoresis is employed in . I. Separation of DNA fragmetns . II. Selection of DNA fragments . III. Vissualizing DNA IV. Check the progression of restriction enzyme digestion.

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    Separation of DNA fragments using agarose gel electrophoresis occurs due to:

    The most commonly used matrix in gel electrophoresis is agarose, which is a natural polymer extracted from :-

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