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Polymerase chain reaction...

Polymerase chain reaction

A

Is a method of synthesising human protein from human DNA

B

Uses restriction enzymes

C

Can produce billions of copies of a DNA fragment

D

Takes place naturally in bacteria

Text Solution

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The correct Answer is:
C
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Study the following columns and choose the correct option {:(Column I,Column II),(A."Synthetic seeds",1."Anther culture"),(B."Gene cloning",2."Interspecific hybridization"),(C."Haploid plants",3."Polymerase chain reaction"),(D."Trasgenic plants",4."Recombinant DNA technology"),(,5."Somatic embryogenesis"):}

A schematic representation of polymerase chain reaction (PCR) up to the extension stage is given below. Which of the following has popularized the PCR (Polymerase Chain Reaction) ? a) Easy availability of DNA template b) Availability of synthetic primers c) Availability of cheap deoxyribonucleotides d) Availability of thermostable DNA polymerase.

(i) Mention the number of primers required in each cycle of polymerase chain reaction (PCR). Write the role of primers and DNA polymerase in PCR. (ii) Give the characteristic feature and source organism of the DNA polymeras, in PCR.

The polymerase chain reaction (PCR) is a noval test tbe method for amplifying first invented in 1984 by kary mullis permits the synthesis of millions of copies of specific DNA sequence in a few hours. Kary mullis (US) and michael smith (british-canadian) got Nobel prize in 1993 in

Match the scientists in column I with their related discoveries in column II and select the correct option from the given codes. {:(ColumnI, Column II),(A."Kary Mullis",(i)"Father of genetic engineering"),(B."Paul Berg",(ii)"Nobel prize for the discovery of restriction endonucleases"),(C."Stanley Cohen and Herbert Boyer",(iii)"Developed polymerase chain reaction"),(D."Arber smith and Nathan",(iv)"Isolated an antibiotic resistance gene from a plasmid of the bacterium Salmonella typhimurium"):}

Which of the following statement // s regarding nucleic acids is // are wrong ? Amplification of gene of interest is done by polymerase Chain Reaction. Origin of replication (ori) is a sequence from where RNA replication starts. The tumour inducing (Ti) plasmid of Agrobacterium tumifaciens can be modified into a cloning vector . DNA technology involves the use of restriction endonucleases. Biolistics or gene gun is suitable to introduce DNA to plant cells.

DINESH PUBLICATION-PRINCIPLES AND APPLICATIONS OF BIOTECHNOLOGY-RQ
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  7. Isolation of DNA from a fungal cell involves the use of enzyme

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  8. The first nif genes were isolated from

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