A: Selection of recombinants due to inactivation of antibiotics is cumbersome procedure.
R: It requires simultaneous plating on two plates having different antibiotics.

Assertion: Selection of recombinants due to inactivation of antibiotic is a cumbersome procedure. Reason: It requires simultaneous planting on two plates having different antibiotics.

In a bacterial culture, some of the colonies produced blue colour in the presence of a chromogenic substrate and some did not due to the presence or absence of an insert (rDNA) in the coding sequence of beta - galactosidase. (a) Mention the mechanism and the steps involved in this experiment. (b) How is it advantageous over simultaneous plating on two plates having different antibiotics?

In bacterial culture some of the colonies produced blue colour in the presence of chromogenic substrate and some did not due to presence or absence of an inert (rDNA) in the coding sequence of B galactosidase. (a) Mention the mechanism and steps involved in above experiment. (b) How is it advantageous over simultaneous plating on two plates having different antibiotics.

For selection of recombinants, insertional inactivation of antibiotic marker has been superseded by insertional inactivation of a marker gene coding for a chromogenic substrate. Give reasons.

For selection of recombinants, insertional inactivation of antibiotic marker has been superseded by insertional inactivation of a marker gene coding for a chromogenic substrate. Give reasons.

For selection of recombinants, insertional inactivation of antibiotic marker has been supercoded by insertional inactivation of a marker gene coding for a chromogenic substrate. Give reasons.