In the human genome project, the method that is involved in identifying all the genes that are expressed as RNA is referred to as

Options

A. sequence annotation.
B. expressed sequence tags.
C. bioinformatics .
D. DNA fingerprinting

Answer

To solve the question regarding the method used in the Human Genome Project to identify all genes expressed as RNA, we can follow these steps:

### Step-by-Step Solution:

1. **Understand the Question**: The question asks for a specific method used in the Human Genome Project that identifies genes expressed as RNA.

2. **Analyze the Options**:
- **Option 1: Sequence Annotation**: This method involves identifying the locations of genes in the genome, but it does not specifically focus on RNA expression. Therefore, this option is incorrect.

- **Option 2: Expressed Sequence Tags (ESTs)**: This method involves the use of short subsequences of cDNA (complementary DNA) to identify gene transcripts. Since ESTs are directly related to identifying genes that are expressed as RNA, this option is likely correct.

- **Option 3: Bioinformatics**: While bioinformatics is crucial for analyzing biological data, it is a broader field that encompasses various tools and methods, not specifically focused on identifying RNA expression. Thus, this option is not the direct answer to the question.

- **Option 4: DNA Fingerprinting**: This technique is used for identifying individuals based on their unique DNA patterns and does not relate to RNA expression. Therefore, this option is also incorrect.

3. **Identify the Correct Answer**: Based on the analysis, the method that specifically identifies genes expressed as RNA is **Expressed Sequence Tags (ESTs)**.

### Final Answer:
The method involved in identifying all the genes that are expressed as RNA in the Human Genome Project is referred to as **Expressed Sequence Tags (ESTs)**.

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