The internodal segments of tobacco stem the callus proliferated only if it contains ……..(i)………in addition to ………..(ii)……..

Options

A. (i) Yeast extract , (ii) coconut milk
B. (i) Coconut milk , (ii) Auxin
C. (i) yeast extract , (ii) cytokinin
D. (i) coconut milk , (ii) Gibberellic acid

Answer

To solve the question regarding the internodal segments of the tobacco stem and the conditions for callus proliferation, we can follow these steps:

### Step-by-Step Solution:

1. **Understanding Callus Formation**:
- Callus is a mass of undifferentiated cells that forms at the site of injury or in tissue culture. It is essential for plant regeneration and growth.

2. **Identifying the Required Hormones**:
- Callus proliferation typically requires specific plant hormones. The two main hormones involved in this process are **auxin** and **cytokinin**.
- Auxin promotes cell elongation and is crucial for root formation.
- Cytokinin promotes cell division and is essential for shoot formation.

3. **Analyzing the Options**:
- The question asks for two components that must be present for the callus to proliferate.
- The first component is likely to be **auxin** since it is critical for callus formation.
- The second component must be **cytokinin**, as it works in conjunction with auxin to promote cell division and growth.

4. **Conclusion**:
- Therefore, the answer to the question is: The internodal segments of tobacco stem callus proliferate only if it contains **auxin** (i) in addition to **cytokinin** (ii).

### Final Answer:
The internodal segments of tobacco stem callus proliferate only if it contains **auxin** in addition to **cytokinin**.

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