Select the option which is not correct with respect to enzyme action.

Options

A. Substrate binds with enzyme at its active site to form enzyme substrate complex.
B. Addition of lot of succinate does not reverse the inhibition of succinic dehydrogenase by malonate.
C. A noncompetitive inhibitor cannot bind the active site of the enzyme at a site distinct from that which binds the substrate .
D. Malonate is a competitive inhibitor of succinic dehydrogenase.

Answer

To solve the question, we need to analyze each of the provided options regarding enzyme action and identify which one is incorrect.

### Step-by-Step Solution:

1. **Understanding Enzyme Action**: Enzymes are biological catalysts that speed up reactions by lowering the activation energy. They have specific sites called active sites where substrates bind to form an enzyme-substrate complex.

2. **Analyzing the Options**:
- **Option 1**: "Substrate binds with enzyme at its active site to form enzyme-substrate complex."
- This statement is correct. The substrate indeed binds to the active site of the enzyme to form an enzyme-substrate complex.

- **Option 2**: "Addition of lot of succinate does not reverse the inhibition of succinic dehydrogenase by malonate."
- This statement is incorrect. Malonate is a competitive inhibitor of succinic dehydrogenase. In competitive inhibition, increasing the concentration of the substrate (succinate) can overcome the inhibition caused by malonate. Therefore, this statement does not hold true.

- **Option 3**: "A non-competitive inhibitor cannot bind the active site of the enzyme at a site distinct from that which binds the substrate."
- This statement is correct. Non-competitive inhibitors bind to an enzyme at a site other than the active site, which does not prevent substrate binding but inhibits the enzyme's activity.

- **Option 4**: "Malonate is a competitive inhibitor of succinic dehydrogenase."
- This statement is also correct. Malonate competes with succinate for the active site of succinic dehydrogenase.

3. **Conclusion**: The option that is not correct with respect to enzyme action is **Option 2**: "Addition of lot of succinate does not reverse the inhibition of succinic dehydrogenase by malonate."

### Final Answer:
**Option 2** is the incorrect statement regarding enzyme action.

---