Which one of the following restriction enzyme can cut `"Tet"^R` site of pBR322 ?

Options

A. Pvu I, Pst I
B. BamH I, Sal I
C. Pvu II
D. EcoR I

Answer

To determine which restriction enzyme can cut the "Tet"^R site of pBR322, we need to analyze the options provided and understand the structure of the pBR322 plasmid.

### Step-by-Step Solution:

1. **Understanding pBR322**:
- pBR322 is an artificial plasmid that contains genes for antibiotic resistance, specifically for tetracycline (Tet^R) and ampicillin (Amp^R). It was constructed by Bolivar and Rodriguez.

2. **Identifying the Tetracycline Resistance Gene**:
- The tetracycline resistance gene (Tet^R) is located at a specific site on the pBR322 plasmid.

3. **Recognizing Restriction Enzymes**:
- Restriction enzymes are proteins that cut DNA at specific sequences. Each enzyme recognizes a unique sequence of nucleotides.

4. **Finding the Relevant Restriction Sites**:
- According to the information provided, the restriction enzymes that can cut the tetracycline site include BAMH1 and SAL1. These enzymes have specific recognition sites that overlap with the Tet^R gene.

5. **Evaluating the Options**:
- If the options provided include BAMH1 and SAL1, then either of these would be the correct answer as they can cleave the Tet^R site on the pBR322 plasmid.

6. **Conclusion**:
- Based on the analysis, the restriction enzyme that can cut the "Tet"^R site of pBR322 is **BAMH1** (assuming it is one of the options provided).

### Final Answer:
The restriction enzyme that can cut the "Tet"^R site of pBR322 is **BAMH1**.

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