GAATTC is a recognition site for the restriction endonuclease

Options

A. EcoRI
B. EcoRII
C. Hindlll
D. BamHI

Answer

To solve the question regarding the recognition site GAATTC for a restriction endonuclease, we can follow these steps:

### Step-by-Step Solution:

1. **Understand the Concept of Restriction Endonucleases**:
- Restriction endonucleases are enzymes that cut DNA at specific sequences called recognition sites. They are crucial in biotechnology for DNA manipulation.

**Hint**: Remember that restriction endonucleases are specific to certain DNA sequences.

2. **Identify the Given Sequence**:
- The sequence provided in the question is GAATTC. We need to determine which restriction enzyme recognizes this sequence.

**Hint**: Look for common restriction enzymes that have known recognition sequences.

3. **Recall Known Restriction Enzymes**:
- One of the most well-known restriction enzymes is EcoRI, which is derived from the bacterium Escherichia coli.

**Hint**: Think about the naming conventions of restriction enzymes; they often include the organism's name.

4. **Check the Recognition Site for EcoRI**:
- The recognition site for EcoRI is indeed GAATTC. This enzyme cuts between the G and the A on both strands, resulting in sticky ends.

**Hint**: Palindromic sequences are often recognized by restriction enzymes; check if GAATTC reads the same in reverse on the complementary strand.

5. **Conclusion**:
- Since GAATTC is the recognition site for EcoRI, we can conclude that the answer to the question is EcoRI.

**Hint**: Always verify the recognition sequence with a reliable source or database if you're unsure.

### Final Answer:
The recognition site GAATTC is associated with the restriction endonuclease EcoRI.