In which region of pBR322 , 5' GAATTC 3' sequence will be present ?

Options

A. rop
B. `tet^R`
C. `amp^R`
D. none of these

Answer

To determine the region of the pBR322 vector where the 5' GAATTC 3' sequence is present, we need to follow these steps:

### Step 1: Understand the pBR322 Vector
- pBR322 is a plasmid vector commonly used in molecular biology, particularly in cloning and gene manipulation. It contains several important regions, including antibiotic resistance genes and multiple cloning sites (MCS).

**Hint:** Familiarize yourself with the structure and function of plasmid vectors like pBR322.

### Step 2: Identify the Sequence
- The sequence 5' GAATTC 3' is recognized by the restriction enzyme EcoRI. This enzyme is known for cutting DNA at this specific palindromic sequence.

**Hint:** Remember that restriction enzymes have specific recognition sequences that they cut.

### Step 3: Analyze the Options
- The question provides different regions of the pBR322 vector. We need to check if any of these regions contain the EcoRI recognition site (GAATTC).

**Hint:** Look closely at the regions mentioned in the options and compare them to the EcoRI recognition sequence.

### Step 4: Determine the Location
- After analyzing the options, it becomes clear that none of the regions mentioned (ROP, antibiotic resistance genes) contain the EcoRI recognition site. The EcoRI site is typically found in the multiple cloning site (MCS) of the vector, which is not listed in the options provided.

**Hint:** Recall that the MCS is where various restriction sites are located for cloning purposes.

### Step 5: Conclusion
- Since the 5' GAATTC 3' sequence is the recognition site for EcoRI and is not found in the provided options, the correct answer is that it is not present in any of the specified regions.

**Final Answer:** None of these (the EcoRI site is not in the given options).