During protein synthesis which of the following is TRUE?

Options

A. `Mn^(++)` is required for completion of ribosome
B. 3' of mRNA enters the ribosome
C. 23s rRNA in bacteria is the enzyme ribozyme that act as catalyst for the formation of peptide bond.
D. EF- Ts helps on codon-anticodon recognition.

Answer

To solve the question "During protein synthesis which of the following is TRUE?", we will analyze each of the provided options step by step.

### Step 1: Analyze Option 1
**Statement**: Manganese is required for the completion of ribosome.
**Evaluation**: This statement is incorrect. The correct ion required for the completion of ribosomes is magnesium, not manganese.

**Hint**: Remember that magnesium plays a crucial role in ribosome assembly.

### Step 2: Analyze Option 2
**Statement**: The 3' end of mRNA enters the ribosome.
**Evaluation**: This statement is also incorrect. The 5' end of mRNA is the one that enters the ribosome first during translation.

**Hint**: Consider the directionality of mRNA and how it interacts with the ribosome.

### Step 3: Analyze Option 3
**Statement**: 23S rRNA, which is ribosomal RNA in bacteria, is the enzyme ribozyme that acts as the catalyst for the formation of peptide bonds.
**Evaluation**: This statement is correct. The 23S rRNA in the large subunit of the bacterial ribosome does indeed function as a ribozyme, catalyzing the formation of peptide bonds between amino acids during protein synthesis.

**Hint**: Recall the role of rRNA in the ribosome and its catalytic functions.

### Step 4: Analyze Option 4
**Statement**: tRNA helps in codon-anticodon recognition.
**Evaluation**: This statement is incorrect as it is misleading. While tRNA does play a role in matching codons with anticodons, it is not the tRNA itself that "helps" in the recognition; rather, it is the complementary base pairing that occurs between the codon on mRNA and the anticodon on tRNA.

**Hint**: Focus on the base pairing mechanism between mRNA and tRNA.

### Conclusion
After evaluating all the options, the correct answer is **Option 3**: 23S rRNA in bacteria acts as a ribozyme that catalyzes the formation of peptide bonds during protein synthesis.