Which of the following statements are false regarding 'Hershey and Chase' experiment, proving DNA is genetic material ?
(i) They made use of rats.
(ii) Radioactivity was seen in supernatant for the medium that was infected by phase radiolabeled with `.^(32)P`.
(iii) Used radioisotope to label sulphur with `.^(35)S` in sulphur- containing amino acids.
(iv) Radioactive phages were allowed to attach to E. coli bacteria.

Options

A. (ii) and (iii) only
B. (iii) and (iv)
C. (ii) , (iii) and (iv) only
D. (i) and (ii)

Answer

To determine which statements are false regarding the Hershey and Chase experiment, we will analyze each statement one by one based on the known facts about the experiment.

### Step-by-Step Solution:

1. **Understanding the Hershey and Chase Experiment**:
- The Hershey and Chase experiment was conducted to determine whether DNA or protein is the genetic material in bacteriophages (viruses that infect bacteria).
- They used two isotopes to label different components of the bacteriophage: phosphorus-32 (for DNA) and sulfur-35 (for proteins).

2. **Analyzing Each Statement**:
- **Statement (i)**: "They made use of rats."
- **Analysis**: This statement is false. Hershey and Chase used bacteriophages and E. coli bacteria, not rats. Therefore, this statement is incorrect.

- **Statement (ii)**: "Radioactivity was seen in supernatant for the medium that was infected by phage radiolabeled with 32P."
- **Analysis**: This statement is also false. In the experiment, when the phages labeled with 32P (which labels DNA) infected the bacteria, the radioactivity was found in the bacterial pellet, not in the supernatant. The supernatant contained the leftover phage proteins labeled with 35S. Thus, this statement is incorrect.

- **Statement (iii)**: "Used radioisotope to label sulfur with 35S in sulfur-containing amino acids."
- **Analysis**: This statement is true. Hershey and Chase used 35S to label the proteins of the bacteriophage, which contain sulfur. Therefore, this statement is correct.

- **Statement (iv)**: "Radioactive phages were allowed to attach to E. coli bacteria."
- **Analysis**: This statement is true. The experiment involved allowing the radioactive phages to attach to E. coli, which is a crucial part of the experiment to demonstrate that DNA is the genetic material. Thus, this statement is correct.

3. **Conclusion**:
- The false statements regarding the Hershey and Chase experiment are:
- (i) They made use of rats.
- (ii) Radioactivity was seen in supernatant for the medium that was infected by phage radiolabeled with 32P.
- Therefore, the correct answer to the question is that statements (i) and (ii) are false.

### Final Answer:
The false statements regarding the Hershey and Chase experiment are (i) and (ii).