Given below are the steps of DNA fingerprinting in random sequence:-
(i) Isolation of DNA.
(ii) Separation of DNA fragements by electrophoresis,
(iii) Hybridisation using labelled VNTR probe. ltBrgt (iv). Transferring of separeted DNA fragments to synthetic membranes, such as nitrocellulose or nylon.
(v) digestion of DNA by restriction endonucleases,
(vi). Detection of hybridised DNA fragments by auto radio-graphy.
which of the following is the correct sequence:-

Describe the technique of separation of DNA fragments by electrophoresis.

The technique of transferring DNA fragment separated on agarose gel to a synthetic membrane like nitrocellulose is known as

Select the correct sequences of step in DNA finger printing involving Southern blot hybridisation using radiolabelled VNTR as probe. I. Hybridisation using labelled VNTR prode. II. Isolation of DNA. III. Transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon. IV. Detection of hybridisation DNA fragments by autoradigraphy. V. Separation of DNA fragments by electrophoresis. VI. Digestion of DNA by restriction endonucleases.

The cutting of DNA by restriction endonucleases results in the fragments of DNA. These fragments can be separated by a technique known as

The cutting of DNA by restriction endonucleases results in the fragments of DNA. These fragments can be separated by a technique known as

Transfer of DNA fragments from the agarose gel to a nylon membrane.

(a) Restriction endonuclease : fragmentation of DNA (b) DNA ligase : _____