Using imprints from a plate with complete medium and carrying bacterial colonies, you can select streptomycin resistant mutants and prove that such mutations do not originate as adaptation. These imprints need to be used

Options

A. on plates with and without streptomycin
B. on plates with minimal medium
C. only on plates with streptomycin
D. only on plates without streptomycin

Explanation

To select streptomycin-resistant mutants from bacterial colonies and prove that such mutations do not originate as adaptations, follow these steps:

### Step-by-Step Solution:

1. **Prepare the Plates**: Start with a plate containing a complete medium where bacterial colonies are already growing. This medium should support the growth of bacteria.

2. **Introduce Streptomycin**: Prepare another plate that contains the same complete medium but with the addition of streptomycin, an antibiotic that inhibits the growth of most bacteria.

3. **Transfer Imprints**: Use imprints from the plate with bacterial colonies to transfer the bacteria onto the streptomycin-containing plate. This can be done using sterile techniques to avoid contamination.

4. **Incubate the Plates**: Allow both plates (the complete medium plate and the streptomycin plate) to incubate under appropriate conditions (temperature, humidity, etc.) to promote bacterial growth.

5. **Observe Growth**: After incubation, observe the plates. On the streptomycin plate, only the bacteria that have developed resistance to streptomycin will survive and grow. The non-resistant bacteria will be inhibited or killed by the antibiotic.

6. **Select Resistant Colonies**: Identify and select the colonies that have grown on the streptomycin plate. These colonies are your streptomycin-resistant mutants.

7. **Prove Mutation Origin**: To demonstrate that these mutations are not adaptations, you can compare the growth of these mutants on a plate without streptomycin. If they grow equally well in both environments, it suggests that the mutations occurred independently of the selective pressure of the antibiotic.