Select the incorrect statement about in-vitro germination of pollen.

Options

A. It can be easily studied by dusting some pollen from flowers such as balsam on a glass slide.
B. Germination generally requires about 45 - 60 minutes.
C. 10% sucrose solution is required for the germination
D. Both (B) and (C )

Answer

To solve the question regarding the incorrect statement about in-vitro germination of pollen, we will analyze each option step by step.

### Step 1: Analyze the First Option
**Statement:** "It can be easily studied by dusting some pollen from the flowers such as balsam on the glass slide."

**Analysis:** This statement is correct. In vitro germination of pollen can indeed be studied by placing pollen on a glass slide, as this allows for observation of the germination process.

**Conclusion:** This option is not the incorrect statement.

### Step 2: Analyze the Second Option
**Statement:** "Germination generally requires about 45 to 60 minutes."

**Analysis:** This statement is incorrect. In vitro pollen germination typically requires a longer duration, often several hours to a day, depending on the species and conditions.

**Conclusion:** This option is the incorrect statement.

### Step 3: Analyze the Third Option
**Statement:** "10% sucrose solution is required for the germination."

**Analysis:** This statement is correct. A 10% sucrose solution is commonly used in in vitro germination as it provides the necessary nutrients for pollen tube growth.

**Conclusion:** This option is not the incorrect statement.

### Step 4: Analyze the Fourth Option
**Statement:** "Both b and c."

**Analysis:** Since option b is incorrect and option c is correct, this statement is also incorrect because it claims both statements are wrong.

**Conclusion:** This option is also not the correct answer.

### Final Conclusion
The incorrect statement about in-vitro germination of pollen is **option 2**: "Germination generally requires about 45 to 60 minutes."

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