You discovered a novel eukaryotic organism that glows in the dark. You believe this trait is due to a single gene, and you wish to clone the gene. Which of the following strategies is most likely to be successful ?

Options

A. Isolate the genomic DNa from the organism, digest with a restriction endonuclease, insert into a plasmid vector and transform into bacteria. Screen colonies for the ability to glow in the dark
B. Isolate the genomic DNA from the organism, digest with a restriction endonuclease, insert into a plasmid vector and transform into eukaryotic cells such as yeast. Screen colonies for the ability to glow in the dark .
C. Isolate the genomic mRNA from the organism, reverse transcribe and generate cDNA, insert into a plasmid vector and transform into bacteria. Screen colonies for the ability to glow in the dark .
D. Isolate the genomic mRNA from the organism, reverse transcribe and generate cDNA, insert into a plasmid vector and transform into eukaryotic cells such as yeast. Screen colonies for the ability to glow in the dark .

Answer

D