A scientist wants to insert a modified DNA directly into the nucleus of the animal cell . Which of the techniques will be best suited ?

Options

A. Gene gun
B. Disarmed pathogen
C. Cloning vector
D. Microinjection

Answer

To solve the question of which technique is best suited for inserting modified DNA directly into the nucleus of an animal cell, we can follow these steps:

### Step 1: Understand the Techniques
We have several techniques mentioned: Gene Gun, Disarmed Pathogen, Cloning Vector, and Micro Injection. We need to evaluate each one based on their applicability to animal cells.

### Step 2: Evaluate Each Technique
1. **Gene Gun**: This method is primarily used for plant cells, where DNA is delivered using high-velocity microprojectiles. It is not suitable for animal cells.

2. **Disarmed Pathogen**: This technique involves using a modified virus to deliver DNA into host cells. While it can be effective, it does not involve direct injection into the nucleus.

3. **Cloning Vector**: This refers to a method where DNA is inserted into a vector (like a plasmid) that can replicate within a host. This method does not directly inject DNA into the nucleus.

4. **Micro Injection**: This technique involves using a fine needle to directly inject DNA into the nucleus of an animal cell. It is a direct method of gene transfer and is commonly used in animal cell manipulation.

### Step 3: Identify the Best Technique
From the evaluation, the only method that directly injects modified DNA into the nucleus of an animal cell is **Micro Injection**. Therefore, this is the best-suited technique for the scientist's purpose.

### Conclusion
The best technique for inserting modified DNA directly into the nucleus of an animal cell is **Micro Injection**.

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