Which is responsible for controlling the...

Which is responsible for controlling the copy number of the linked DNA in a plasmid ?

Selectable marker

Ori site

Palindromic sequence

Recognition site

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Assertion : If one wants to recover many copies of the target DNA it should be cloned in a vector whose origin support high copy number Reason: Ori is responsible for controlling the copy number of the linked DNA/target DNA

DNA of plasmid is

(i) Name the organism in which the vector shown in inserted to get the copies of the desired gene. (ii) Mention the area labelled in the vector responsible for controlling the copy number of the inserted gene. (iii) Name and explain the role of a selectable marker in the vector shown.

Which of the following statements are correct ? (i) Restriction enzymes cut the strand of DNA a little away from the centre of the palindrome site, but between the same two bases on the opposite strands. (ii) Hind ll always cuts DNA molecules at a particular point by reconginisng a specific sequence of six base pairs. (iii) Separated DNA fragments cannot be visualised without staining on an agarose gel electrophoresis. (iv) 'Ori' is the sequence responsible for controlling the copy number. (v) DNA is a positively charged molecule.

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