For the extraction of biomolecule from living tissue. This tissue has to be grind in which chemical?

Answer

To extract biomolecules from living tissue, the following steps are typically followed:

### Step-by-Step Solution:

1. **Preparation of Tissue Sample**: Start by obtaining the living tissue from which you want to extract the biomolecules. This could be any biological tissue such as plant leaves, animal organs, etc.

**Hint**: Ensure that the tissue is fresh and properly handled to maintain the integrity of the biomolecules.

2. **Grinding the Tissue**: The next step is to grind the tissue. This is done to break down the cellular structure and release the biomolecules into solution.

**Hint**: Use a mortar and pestle or a homogenizer for effective grinding.

3. **Adding Tri-chloroacetic Acid (TCA)**: While grinding the tissue, add tri-chloroacetic acid (TCA) to the mixture. TCA is a strong acid that helps in precipitating proteins and extracting various biomolecules.

**Hint**: TCA is chemically represented as C3HCl3O2. Make sure to use it in appropriate concentrations to avoid damaging the biomolecules.

4. **Creating a Paste**: Continue grinding until you achieve a uniform paste. This paste will contain the biomolecules suspended in the TCA solution.

**Hint**: The consistency of the paste should be smooth, without large chunks of tissue remaining.

5. **Separation of Solutions**: After grinding, you will have two distinct solutions:
- **Acid Soluble Pool**: This contains low molecular weight cytoplasmic constituents (less than 800 Dalton).
- **Acid Insoluble Pool**: This contains larger molecules and organelles that do not dissolve in TCA.

**Hint**: You can separate these pools by centrifugation, where the heavier components will settle at the bottom.

6. **Analysis of Extracted Biomolecules**: Finally, analyze the two pools for the biomolecules of interest, which may include proteins, nucleic acids, or other metabolites.

**Hint**: Use appropriate biochemical assays or techniques like chromatography or electrophoresis for analysis.

### Summary:
To extract biomolecules from living tissue, grind the tissue in tri-chloroacetic acid (TCA) to create a paste, which will allow for the separation of soluble and insoluble biomolecules.