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Explain Hershey-Chase experiment. What w...

Explain Hershey-Chase experiment. What was proved through this experiment ?

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Hershey and Chase (1952) conducted their experiment on `T_2` phage which attacks the bacterium E. coli.
The phage particles were prepared by using radioisotopes of `.^35S "and ".^32P`
Conclusion from the Experment : (i) The bacteria which were infected with viruses that had radioactive DNA were radioactive.
(ii) In the phage particles with radioactive protein, the bacterial pellets have almost nil, radioactivity indicating that protein have failed to migrate into bacterial all. So, it wag concluded that DNA must have entered the bacteria and that DNA is the genetic material.
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Knowledge Check

  • Hershey and Chase's experiment was based on the principle:

    A
    Transformation
    B
    Translation
    C
    Transduction
    D
    Transcription

  • Swan neck flask experiment proved :

    A
    biogenesis
    B
    abiogenesis
    C
    special creation
    D
    both (1) and (2)

  • Hershey-Chase experiment successfully proved DNA to be genetic material. The correct sequence of steps followed in this experiment is

    A
    Blending, Infection, Centrifugation
    B
    Centrifugation, Infection, Blending
    C
    Infection, Centrifugation, Blending
    D
    Infection, Blending, Centrifugation.

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    You are repeating the Hershey-Chase experiment and are provided with two isotopes .^(32)Pand .^(15)N (in place of .^(35)S in the original experiment ). How does yoe expect your results to be differnet?

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