State how has Agrobacterium tumefaciens been made a useful cloning vector to transfer DNA to plant cells.

Why is Agrobacterium tumefaciens a good cloning vector ? Explain.

How has Agrobacterium tumefaciens has been suitably modified to act as cloning vector?

Agrobacterium tumefaciens is most widely used for gene transfer bacause of

Read the following statement and choose the correct option: I. Agrobacterium tumerfaciencs, a pathogen of several monocot plants, is able to deliver a piece of DNA known as "T-DNA". II. The tumor inducing (Ti) plasmid of Agrobacterium tumefaciens has been modified into a cloning vector, which is more pathogenic to the plants.

Which of the following statement // s regarding nucleic acids is // are wrong ? Amplification of gene of interest is done by polymerase Chain Reaction. Origin of replication (ori) is a sequence from where RNA replication starts. The tumour inducing (Ti) plasmid of Agrobacterium tumifaciens can be modified into a cloning vector . DNA technology involves the use of restriction endonucleases. Biolistics or gene gun is suitable to introduce DNA to plant cells.

Assertion (A) : Ti plasmid (tumour-inducing plasmid) of Agrobacterium tumefaciens is used as a cloning vector. Reason (R): Ti plasmid integrates a segment of its DNA, termed T-DNA into the chromosomal DNA of its host plant cells.

Assertion : Agrobacterium tumefaciencs causes crown gall tumor in plants. Reason : E.coli can't transfer DNA in plants.