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State the functions of the following in ...

State the functions of the following in the cloning vector pBR322 :
(i) Ori,
(ii) rop, and
(iii) Hind III sites.

Text Solution

Verified by Experts

Functions of :
(1) Ori-Ori is a sequence from where replication starts and any piece of DNA when linked to this sequence can be made to replicate within the host cells.
(2) rop-It codes for the proteins involved in the replication of the plasmid.
(3) Hind III sites-Their function is to protect the host genome against invasion by foreign . DNA. It is the DNA restriction enzymes.
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