State the functions of the following in the cloning vector pBR322 : (i) Ori, (ii) rop, and (iii) Hind III sites.
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Functions of : (1) Ori-Ori is a sequence from where replication starts and any piece of DNA when linked to this sequence can be made to replicate within the host cells. (2) rop-It codes for the proteins involved in the replication of the plasmid. (3) Hind III sites-Their function is to protect the host genome against invasion by foreign . DNA. It is the DNA restriction enzymes.
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