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Mention the role of (i) selectable marke...

Mention the role of (i) selectable marker, (ii) Ori and (iii) rop in E. coli cloning vector pBR322.

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To answer the question regarding the roles of (i) selectable marker, (ii) Ori, and (iii) Rop in the E. coli cloning vector pBR322, we can break down the explanation into three parts: ### Step-by-Step Solution: 1. **Role of Selectable Marker:** - The selectable markers in pBR322 are the genes that confer resistance to antibiotics, specifically ampicillin and tetracycline. - These markers allow for the selection of transformed E. coli cells that have taken up the plasmid. - When E. coli is grown in the presence of ampicillin or tetracycline, only those cells that contain the plasmid (and thus the resistance genes) will survive, while those without the plasmid will die. ...
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Write the role of 'Ori' and 'restriction' site in a cloning vector pBR322.

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Knowledge Check

  • Selectable markers in PBR322

    A
    `"amp"^(R) & "tet"^(R)`
    B
    ori & rop
    C
    Bam HI & Clal
    D
    PstI & PvuI

  • In E.coli cloning vector PBR 322. ROP codes for -

    A
    The proteins involved in the replication of the plasmid
    B
    The proteins involved in the antibiotic resistance
    C
    The proteins involved in the synthesis of antibiotic
    D
    All of these

  • Identify A,B,C,D in the given diagram of E.coli cloning vector pBR322.

    A
    `{:(A" "B" "C" "D),("Hindi 1"" ""EcoR1"" "amp^(R)" ""ori"):}`
    B
    `{:(A" "B" "C" "D),("Hindi 1"" ""BamH1"" "kan^(R)" "amp^(R)):}`
    C
    `{:(A" "B" "C" "D),("BamH1"" ""Pst 1"" "Ori" "amp^(R)):}`
    D
    `{:(A" "B" "C" "D),("EcoR 1"" ""BamH1"" "amp^(R)" ""ori"):}`

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    Read the following statements and select the correct option. (I) E.coli cloning vector pBR322 have several restriction sites , ori , antibiotic resistance genes and rop. (II) Competent bacterial cells cannot take up foreign DNA. (III) In elution ,the separated bands of DNA are cut out fron agarose gel and extracted from the gel piece. (IV) The downstream processing and quality control testing vary from product to product .

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