Mention the role of (i) selectable marker, (ii) Ori and (iii) rop in E. coli cloning vector pBR322.

Explanation

To answer the question regarding the roles of (i) selectable marker, (ii) Ori, and (iii) Rop in the E. coli cloning vector pBR322, we can break down the explanation into three parts:

### Step-by-Step Solution:

1. **Role of Selectable Marker:**
- The selectable markers in pBR322 are the genes that confer resistance to antibiotics, specifically ampicillin and tetracycline.
- These markers allow for the selection of transformed E. coli cells that have taken up the plasmid.
- When E. coli is grown in the presence of ampicillin or tetracycline, only those cells that contain the plasmid (and thus the resistance genes) will survive, while those without the plasmid will die.
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