(a) Name the selectable markers in the cloning vector pBR322 ? Mention the role they play.
(b) Why is the coding sequence of an enzyme b-galactosidase a preferred selectable marker in comparison to the ones named above ?

Name the selectable markers in the cloning vector pBR322? Mention the role of they play.

In the E.coli cloning vector pBR 322, the number of selectable marker is

(a) Identify the selectable maekes in the diagram in the diagram of E. Coli vectior shown above . ( b) How is the coding squence of alpha - galactosides consider a batter markes than the ones identified by you in the diagram ? Explain.

How does beta -galactosidase coding sequence act as a selectable marker ? Why is it a preferred selectable marker to antibiotic resistance genes ? Explain.

In cloning vector pBR322, the Barn HI cloning site is found in which selectable marker gene?

Assertion (A): B-galactosidase coding sequence act as a selectable marker. Reason (R) : Enzyme galactosidase converts the galactose into lactose.

Explain the role(s) of the following Biotechnology: a) Restriction endonuclease Gel-electrophoresis c) Selectable markers in pBR322.

(i) Name the organism in which the vector shown in inserted to get the copies of the desired gene. (ii) Mention the area labelled in the vector responsible for controlling the copy number of the inserted gene. (iii) Name and explain the role of a selectable marker in the vector shown.

In bacterial culture some of the colonies produced blue colour in the presence of chromogenic substrate and some did not due to presence or absence of an inert (rDNA) in the coding sequence of B galactosidase. (a) Mention the mechanism and steps involved in above experiment. (b) How is it advantageous over simultaneous plating on two plates having different antibiotics.