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Mention the number of primers required i...

Mention the number of primers required in each cycle of PCR. Writen the role of primers and DNA polymerase in PCR. Name the source organism of DNA polymerase used in PCR.

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Role of Primers and DNA Polymerase in PCR:
(i) During PCR process in the second stage, each separted DNA stand is allowed to hybridize with a primer. This is called renaturation or primer annealing. The primer template is used to synthesize DNA by using Taq-DNA polymerase.
(ii) Annealing is done by rapid colling of the mixture allowing the primers to bind to the sequences in each of the two strands flanking the target DNA.
(iii) During the third stage known as primer extension or synthesis, the Taq DNA polymerase extends each primer by copying the single stranded template.
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