A Danish Physician, Christian Gram developed a staining procedure to differentiate bacteria. List the various steps involved in that procedure.

Explanation

Gram staining Techniques :
1. Prepare a smear of bacterial culture.
2. Stain with crystal violet for 30 seconds.
3. Rinse in distilled water for 2 seconds.
4. Grams Iodine for 1 minute.
5. Rinse in distilled water.
6. Wash in 95% ethanol or acetone for 10 to 30 seconds.
7. Rinse in distilled water .
8. Safranin for 30-60 seconds .
9. Rinse in distilled water and blot .
10. Observe under microscope.