What is tissue culture? Mention any four application.

Explanation

Separation of cell or tissue from plants and growing them on a sytnetic nutrient medium under aseptic conditions is called tissue. Culture.
involves the following step:
(1). Selection of plant material: The part of the plant unsed for culture is known as explant. it is isolated from leaves, seeds, roots, buds, nodes etc.
Inoculation: It is the process of transfer of explants on the sterlised culture medium. it is done in a special chamber called lamina air flow table.
Advantages: ,br> (1). Androgenic haploid plants: Haploid plants can be produced through anther or pollen culture. diploid homozygous plants can be produced from the halpoid plants by doubling the chromosome number.
(2) Induction and selection of mutants:
Mutation are induced int he cell cultures, and the mutants are then subjected to herbicides, toxins etc. the mutant cells that show resistance are selected and grown by tissue culture to raise the resistance varieties.